"Differentiating T-Cells from hiPSCs to Create Off-The-Shelf SPEAR T-Cell Therapies"
als mündliche Präsentation Nummer 990
"Unedited hiPSCs can express either αβ or γδ TCR after differentiation;
both subsets show multiple effector functions and release cytokines
(IFNγ and TNFα) and lytic granules (Granzyme A and B, CD107a)
when stimulated. Lentiviral transduction of progenitors with SPEAR
(specific peptide enhanced affinity receptors) promotes surface
expression of CD3 and SPEAR on the differentiated cells, which
show antigen-specific activation. These TCR+ cells show functional
characteristics that make them ideal to produce allogeneic, off-theshelf T-cell therapies for oncology, where consistent batches can be
manufactured to treat multiple patients."